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Effects of isoflavonoids and their metabolites on vascular smooth muscles in vitro and in vivo
Migkos, Thomas ; Mladěnka, Přemysl (advisor) ; Studenik, Christian (referee) ; Trejtnar, František (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Pharmacology & Toxicology Candidate: Thomas Migkos, M.Sc. Supervisor: Prof. Přemysl Mladěnka, Pharm.D, Ph.D. Consultant: Assoc. Prof. Jana Pourová, Pharm.D, Ph.D. Title of dissertation thesis: Effects of isoflavonoids and their metabolites on vascular smooth muscles in vitro and in vivo The dietary intake of flavonoids seems to be inversely related to cardiovascular mortality, in particular on coronary artery disease. The consumption of isoflavonoids, one class of flavonoids, has been increasing in the general population, especially due to the use of food supplements and a variety of isoflavonoid-rich foods. Although their bioavailability is low, they undergo extensive gastrointestinal metabolism by human bacteria, leading to smaller metabolites with a much higher degree of bioavailability. However, detailed studies on the impact of individual pure isoflavonoids on vascular system were mostly missing and much less was known for the effect of their colonic metabolites in this field. In the present study sixteen isoflavonoids, four metabolites and the racemic mixture of one of them were initially screened ex vivo for their vasorelaxant properties on rat aortas. The most potent of them, biochanin A, glycitein, O-desmethylangolensin...
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Copper-chelating properties of isoflavonoids
Horňasová, Veronika ; Mladěnka, Přemysl (advisor) ; Spilková, Jiřina (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Veronika Horňasová Supervisor: Assoc. Prof. Přemysl Mladěnka, Ph.D. Title of diploma thesis: Copper-chelating properties of isoflavonoids Copper is an essential trace element that plays a crucial role in our organism. It is involved in many physiological processes. On the other hand, free copper may be toxic for the organism and therefore copper homeostasis must be carefully controlled. Copper chelators represent a promising component of therapeutic strategies not only in absolute but also in relative (local) excess of copper. Especially substances which are expected to have a lower risk for side effects, such as isoflavones, may be beneficial in the treatment of Wilson's disease as well as neurodegenerative diseases or cancer. Isoflavones belong to one of the subclasses of flavonoids. Their biological effects are quite large - anti-inflammatory, antioxidant, cardio- and neuroprotective and anti-tumor. In this diploma thesis chelating properties of a group of ten isoflavones were tested by two indicators - hematoxylin and bathocuproin. In order to determine the chelating properties, a spectrophotometric measurement was used. It is a precise and rapid method. From the tested compounds,...
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Copper-chelating properties of isoflavonoids
Horňasová, Veronika ; Mladěnka, Přemysl (advisor) ; Spilková, Jiřina (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Veronika Horňasová Supervisor: Assoc. Prof. Přemysl Mladěnka, Ph.D. Title of diploma thesis: Copper-chelating properties of isoflavonoids Copper is an essential trace element that plays a crucial role in our organism. It is involved in many physiological processes. On the other hand, free copper may be toxic for the organism and therefore copper homeostasis must be carefully controlled. Copper chelators represent a promising component of therapeutic strategies not only in absolute but also in relative (local) excess of copper. Especially substances which are expected to have a lower risk for side effects, such as isoflavones, may be beneficial in the treatment of Wilson's disease as well as neurodegenerative diseases or cancer. Isoflavones belong to one of the subclasses of flavonoids. Their biological effects are quite large - anti-inflammatory, antioxidant, cardio- and neuroprotective and anti-tumor. In this diploma thesis chelating properties of a group of ten isoflavones were tested by two indicators - hematoxylin and bathocuproin. In order to determine the chelating properties, a spectrophotometric measurement was used. It is a precise and rapid method. From the tested compounds,...
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